Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PP2AC alpha/beta in NIH3T3 mouse fibroblasts ectopically expressing N-terminally tagged human PP2A catalytic subunits (Courtesy of Dr. Stefan Schuechner and Dr. Egon Ogris, Medical University of Vienna, Austria).

Research CategoryEpigenetics & Nuclear Function

Anti-PP2AC alpha/beta, clone 11H12, Cat. No. MABE1783, is a highly specific mouse monoclonal antibody that targets Serine/threonine-protein phosphatase 2A catalytic subunit alpha and beta isoforms and has been tested for use in Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Serine/threonine-protein phosphatase 2A catalytic subunit alpha/beta isoforms (UniProt: P67775/P62714; also known as EC 3.1.3.16, PP2A-alpha/PP2A-beta) is encoded by the PPP2CA/PPP2CB gene (Gene ID: 5515/5516) in human. Protein phosphatase 2A (PP2A) is a ubiquitously expressed enzyme that contributes 0.3 to 1% of the total cellular protein in the mammalian cell and regulates various functions by dephosphorylating many critical molecules, such as Akt, p53, c-Myc, CK2, S6 kinase, and beta-catenin. It is the major phosphatase for microtubule-associated proteins (MAPs). PP2A acts as a pivotal cellular switch controlling important events in cell, such as cell cycle, apoptosis, and autophagy It suppresses mitogenic signals and is suggested as a tumor suppressor. Aberrant expression and mutations in the PP2AA and PP2AB subunits are reported in various human malignancies. PP2A is made up of a functional heterotrimeric complex, which includes a structural subunit (PP2AA), a regulatory subunit (PP2AB), and a catalytic subunit (PP2AC). The regulatory subunit determines its substrate specificity. Over 20 different interchangeable regulatory subunits have been described that allows it to act on a large number of substrates. PP2AC has multiple metal ion binding sites and binds two manganese ions per subunit. Two isoforms of PP2AC (alpha and beta) have been described that display high degree of homology. PP2AC alpha also has two isoforms (PP2AC alpha 1 and alpha 2). The PP2AC alpha 2 isoform is shown to be catalytically inactive because it lacks amino acids 193-246.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Protein Phosphatase 2A catalytic subunit (PP2AC). The immungen sequence is conserved between alpha and beta isoforms of PP2AC.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PP2AC alpha/beta in A549 cell lysate.

Specificity

Clone 11H12 detects human Serine/threonine-protein phosphatase 2A catalytic subunit alpha/beta isoforms (PP2AC alpha and beta). It targets an epitope within 15 amino acids from the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 35 kDa observed; 35.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).